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Journal articles
Kinky DNA in solution: Small-angle-scattering study of a nucleosome positioning sequence
Abstract : DNA is a flexible molecule, but the degree of its flexibility is subject to debate. The commonly-accepted persistence length of l p ≈ 500 Å is inconsistent with recent studies on short-chain DNA that show much greater flexibility but do not probe its origin. We have performed x-ray and neutron small-angle scattering on a short DNA sequence containing a strong nucleosome positioning element and analyzed the results using a modified Kratky-Porod model to determine possible conformations. Our results support a hypothesis from Crick and Klug in 1975 that some DNA sequences in solution can have sharp kinks, potentially resolving the discrepancy. Our conclusions are supported by measurements on a radiation-damaged sample, where single-strand breaks lead to increased flexibility and by an analysis of data from another sequence, which does not have kinks, but where our method can detect a locally enhanced flexibility due to an AT domain.
Cited literature [35 references]
https://hal-ens-lyon.archives-ouvertes.fr/ensl-01910577
Contributor : Michel Peyrard Connect in order to contact the contributor
Submitted on : Thursday, November 1, 2018 - 12:42:16 PM
Last modification on : Tuesday, May 11, 2021 - 9:54:30 AM
Long-term archiving on: : Saturday, February 2, 2019 - 1:14:57 PM
Contributor : Michel Peyrard Connect in order to contact the contributor
Submitted on : Thursday, November 1, 2018 - 12:42:16 PM
Last modification on : Tuesday, May 11, 2021 - 9:54:30 AM
Long-term archiving on: : Saturday, February 2, 2019 - 1:14:57 PM
File
PhysRevE.98.042417.pdf
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