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Aging Yeast Cells Undergo a Sharp Entry into Senescence Unrelated to the Loss of Mitochondrial Membrane Potential

Abstract : In budding yeast, a mother cell can produce a finite number of daughter cells before it stops dividing and dies. Such entry into senescence is thought to result from a progressive decline in physiological function, including a loss of mitochondrial membrane potential (ΔΨ). Here, we developed a microfluidic device to monitor the dynamics of cell division and ΔΨ in real time at single-cell resolution. We show that cells do not enter senescence gradually but rather undergo an abrupt transition to a slowly dividing state. Moreover, we demonstrate that the decline in ΔΨ, which is observed only in a fraction of cells, is not responsible for entry into senescence. Rather, the loss of ΔΨ is an age-independent and heritable process that leads to clonal senescence and is therefore incompatible with daughter cell rejuvenation. These results emphasize the importance of quantitative single-cell measurements to decipher the causes of cellular aging.
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https://hal-ens-lyon.archives-ouvertes.fr/ensl-01074680
Contributor : Agnès Ganivet Connect in order to contact the contributor
Submitted on : Wednesday, October 15, 2014 - 10:46:30 AM
Last modification on : Friday, November 27, 2020 - 10:20:04 AM

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  • HAL Id : ensl-01074680, version 1

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Steffen Fehrmann, Camille Paoletti, Youlian Goulev, Andrei Ungureanu, Hugo Aguilaniu, et al.. Aging Yeast Cells Undergo a Sharp Entry into Senescence Unrelated to the Loss of Mitochondrial Membrane Potential. Cell Reports, Elsevier Inc, 2013, 5 (6), pp.1589-1599. ⟨ensl-01074680⟩

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