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Journal articles
Aging Yeast Cells Undergo a Sharp Entry into Senescence Unrelated to the Loss of Mitochondrial Membrane Potential
Abstract : In budding yeast, a mother cell can produce a finite number of daughter cells before it stops dividing and dies. Such entry into senescence is thought to result from a progressive decline in physiological function, including a loss of mitochondrial membrane potential (ΔΨ). Here, we developed a microfluidic device to monitor the dynamics of cell division and ΔΨ in real time at single-cell resolution. We show that cells do not enter senescence gradually but rather undergo an abrupt transition to a slowly dividing state. Moreover, we demonstrate that the decline in ΔΨ, which is observed only in a fraction of cells, is not responsible for entry into senescence. Rather, the loss of ΔΨ is an age-independent and heritable process that leads to clonal senescence and is therefore incompatible with daughter cell rejuvenation. These results emphasize the importance of quantitative single-cell measurements to decipher the causes of cellular aging.
https://hal-ens-lyon.archives-ouvertes.fr/ensl-01074680
Contributor : Agnès Ganivet <>
Submitted on : Wednesday, October 15, 2014 - 10:46:30 AM
Last modification on : Tuesday, May 26, 2020 - 11:14:02 PM
Contributor : Agnès Ganivet <>
Submitted on : Wednesday, October 15, 2014 - 10:46:30 AM
Last modification on : Tuesday, May 26, 2020 - 11:14:02 PM