Aging Yeast Cells Undergo a Sharp Entry into Senescence Unrelated to the Loss of Mitochondrial Membrane Potential

Steffen Fehrmann 1 Camille Paoletti 1 Youlian Goulev 1 Andrei Ungureanu 2 Hugo Aguilaniu 3 Gilles Charvin 1
1 IGBMC - Institut de Génétique et de Biologie Moléculaire et Cellulaire
2 Laboratoire Joliot Curie
3 LBMC - Laboratoire de Biologie Moléculaire de la Cellule
Abstract : In budding yeast, a mother cell can produce a finite number of daughter cells before it stops dividing and dies. Such entry into senescence is thought to result from a progressive decline in physiological function, including a loss of mitochondrial membrane potential (ΔΨ). Here, we developed a microfluidic device to monitor the dynamics of cell division and ΔΨ in real time at single-cell resolution. We show that cells do not enter senescence gradually but rather undergo an abrupt transition to a slowly dividing state. Moreover, we demonstrate that the decline in ΔΨ, which is observed only in a fraction of cells, is not responsible for entry into senescence. Rather, the loss of ΔΨ is an age-independent and heritable process that leads to clonal senescence and is therefore incompatible with daughter cell rejuvenation. These results emphasize the importance of quantitative single-cell measurements to decipher the causes of cellular aging.
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Cell Reports, Elsevier (Cell Press), 2013, 5 (6), pp.1589-1599. 〈doi: 10.1016〉
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https://hal-ens-lyon.archives-ouvertes.fr/ensl-01074680
Contributeur : Agnès Ganivet <>
Soumis le : mercredi 15 octobre 2014 - 10:46:30
Dernière modification le : mercredi 14 mars 2018 - 16:48:30

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Steffen Fehrmann, Camille Paoletti, Youlian Goulev, Andrei Ungureanu, Hugo Aguilaniu, et al.. Aging Yeast Cells Undergo a Sharp Entry into Senescence Unrelated to the Loss of Mitochondrial Membrane Potential. Cell Reports, Elsevier (Cell Press), 2013, 5 (6), pp.1589-1599. 〈doi: 10.1016〉. 〈ensl-01074680〉

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