HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Endogenous nuclear RNAi mediates behavioral adaptation to odor

Abstract : Most eukaryotic cells express small regulatory RNAs. The purpose of one class, the somatic endogenous siRNAs (endo-siRNAs), remains unclear. Here, we show that the endo-siRNA pathway promotes odor adaptation in C. elegans AWC olfactory neurons. In adaptation, the nuclear Argonaute NRDE-3, which acts in AWC, is loaded with siRNAs targeting odr-1, a gene whose downregulation is required for adaptation. Concomitant with increased odr-1 siRNA in AWC, we observe increased binding of the HP1 homolog HPL-2 at the odr-1 locus in AWC and reduced odr-1 mRNA in adapted animals. Phosphorylation of HPL-2, an in vitro substrate of the EGL-4 kinase that promotes adaption, is necessary and sufficient for behavioral adaptation. Thus, environmental stimulation amplifies an endo-siRNA negative feedback loop to dynamically repress cognate gene expression and shape behavior. This class of siRNA may act broadly as a rheostat allowing prolonged stimulation to dampen gene expression and promote cellular memory formation
Document type :
Journal articles
Complete list of metadata

https://hal-ens-lyon.archives-ouvertes.fr/ensl-00961451
Contributor : Agnès Ganivet Connect in order to contact the contributor
Submitted on : Thursday, March 20, 2014 - 10:12:27 AM
Last modification on : Thursday, November 21, 2019 - 2:26:27 AM

Identifiers

  • HAL Id : ensl-00961451, version 1
  • PUBMED : 23993094

Collections

Citation

Bi-Tzen Juang, Chen Gu, Linda Starnes, Francesca Palladino, Andrei Goga, et al.. Endogenous nuclear RNAi mediates behavioral adaptation to odor. Cell, Elsevier, 2013, 154 (5), pp.10. ⟨ensl-00961451⟩

Share

Metrics

Record views

27