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Purification of A-form DNA fibre samples by removal of B-form DNA residues

Abstract : To date, fibre diffraction on A-form NaDNA has always shown a B-form contamination. Here, we have used optic microscopy, calorimetry and neutron scattering techniques to define a method to obtain DNA fibres samples whose molecules are purely in the A-form. When the impure sample is heated to 320 K, the DNA molecules in the B-form undergo a transition into the A-form. Our studies have modified the accepted phase diagram for NaDNA films by including the dependence of temperature crucial for the purification of A-form samples by removal of B-form contamination.
keyword : DNA B-A transition
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https://hal-ens-lyon.archives-ouvertes.fr/ensl-00934635
Contributor : Michel Peyrard Connect in order to contact the contributor
Submitted on : Wednesday, January 22, 2014 - 1:46:51 PM
Last modification on : Tuesday, March 8, 2022 - 3:16:08 PM

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Jessica Valle Orero, Andrew Wildes, Jean-Luc Garden, Michel Peyrard. Purification of A-form DNA fibre samples by removal of B-form DNA residues. Journal of Physical Chemistry B, American Chemical Society, 2013, 117, pp.1849-1856. ⟨10.1021/jp311199f⟩. ⟨ensl-00934635⟩

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