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Journal Articles Journal of Physical Chemistry B Year : 2013

Purification of A-form DNA fibre samples by removal of B-form DNA residues

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Abstract

To date, fibre diffraction on A-form NaDNA has always shown a B-form contamination. Here, we have used optic microscopy, calorimetry and neutron scattering techniques to define a method to obtain DNA fibres samples whose molecules are purely in the A-form. When the impure sample is heated to 320 K, the DNA molecules in the B-form undergo a transition into the A-form. Our studies have modified the accepted phase diagram for NaDNA films by including the dependence of temperature crucial for the purification of A-form samples by removal of B-form contamination.
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Dates and versions

ensl-00934635 , version 1 (22-01-2014)

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Jessica Valle Orero, Andrew Wildes, Jean-Luc Garden, Michel Peyrard. Purification of A-form DNA fibre samples by removal of B-form DNA residues. Journal of Physical Chemistry B, 2013, 117, pp.1849-1856. ⟨10.1021/jp311199f⟩. ⟨ensl-00934635⟩
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