Purification of A-form DNA fibre samples by removal of B-form DNA residues

Jessica Valle Orero; Andrew Wildes; Jean-Luc Garden; Michel Peyrard

Purification of A-form DNA fibre samples by removal of B-form DNA residues

Jessica Valle Orero ¹ Andrew Wildes ² Jean-Luc Garden ³ Michel Peyrard ¹

1 Phys-ENS - Laboratoire de Physique de l'ENS Lyon

2 ILL - Institut Laue-Langevin

3 CapTher - Capteurs thermométriques et calorimétrie

NEEL - Institut Néel

Abstract : To date, fibre diffraction on A-form NaDNA has always shown a B-form contamination. Here, we have used optic microscopy, calorimetry and neutron scattering techniques to define a method to obtain DNA fibres samples whose molecules are purely in the A-form. When the impure sample is heated to 320 K, the DNA molecules in the B-form undergo a transition into the A-form. Our studies have modified the accepted phase diagram for NaDNA films by including the dependence of temperature crucial for the purification of A-form samples by removal of B-form contamination.

keyword : DNA B-A transition

Document type :

Journal articles

Domain :

Physics [physics] / Condensed Matter [cond-mat] / Statistical Mechanics [cond-mat.stat-mech]

Complete list of metadatas

https://hal-ens-lyon.archives-ouvertes.fr/ensl-00934635
Contributor : Michel Peyrard <>
Submitted on : Wednesday, January 22, 2014 - 1:46:51 PM
Last modification on : Wednesday, February 13, 2019 - 10:06:07 AM

Purification of A-form DNA fibre samples by removal of B-form DNA residues

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Purification of A-form DNA fibre samples by removal of B-form DNA residues

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