Single-base resolution mapping of H1-nucleosome interactions and 3D organization of the nucleosome

Sajad Hussain Syed 1, 2 Damien Goutte-Gattat 1 Nils Becker 3 Sam Meyer 3 Manu Shubhdarshan Shukla 1, 2 Jeffrey J. Hayes 4 Ralf Everaers 3 Dimitar Angelov 2 Jan Bednar 5, 6 Stefan Dimitrov 1
1 Institut Albert Bonniot
2 LBMC - Laboratoire de Biologie Moléculaire de la Cellule
3 Phys-ENS - Laboratoire de Physique de l'ENS Lyon
4 Department of Biochemistry and Biophysics
5 LSP - Laboratoire de Spectrométrie Physique
6 Institute of Cellular Biology and Pathology
Abstract : Despite the key role of the linker histone H1 in chromatin structure and dynamics, its location and interactions with nucleosomal DNA have not been elucidated. In this work we have used a combination of electron cryomicroscopy, hydroxyl radical footprinting, and nanoscale modeling to analyze the structure of precisely positioned mono-, di-, and trinucleosomes containing physiologically assembled full-length histone H1 or truncated mutants of this protein. Single-base resolution *OH footprinting shows that the globular domain of histone H1 (GH1) interacts with the DNA minor groove located at the center of the nucleosome and contacts a 10-bp region of DNA localized symmetrically with respect to the nucleosomal dyad. In addition, GH1 interacts with and organizes about one helical turn of DNA in each linker region of the nucleosome. We also find that a seven amino acid residue region (121-127) in the COOH terminus of histone H1 was required for the formation of the stem structure of the linker DNA. A molecular model on the basis of these data and coarse-grain DNA mechanics provides novel insights on how the different domains of H1 interact with the nucleosome and predicts a specific H1-mediated stem structure within linker DNA.
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Journal articles
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https://hal-ens-lyon.archives-ouvertes.fr/ensl-00817666
Contributor : Agnès Ganivet <>
Submitted on : Thursday, April 25, 2013 - 10:22:15 AM
Last modification on : Wednesday, November 20, 2019 - 3:22:23 AM

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  • HAL Id : ensl-00817666, version 1
  • DOI : 10.1073

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Sajad Hussain Syed, Damien Goutte-Gattat, Nils Becker, Sam Meyer, Manu Shubhdarshan Shukla, et al.. Single-base resolution mapping of H1-nucleosome interactions and 3D organization of the nucleosome. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2010, 107 (21), pp.9620-9625. ⟨10.1073⟩. ⟨ensl-00817666⟩

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