The human TTAGGG repeat factors 1 and 2 bind to a subset of interstitial telomeric sequences and satellite repeats

Thomas Simonet 1 Laure-Emmanuelle Saragosi 2 Claude Philippe 3 Kevin Lebrigand 2 Clémentine Schouteden 1 Adeline Augereau 1, 3 Serge Bauwens 1 Jing Ye 1, 3 Mario Santagostino 4 Elena Giulotto 4 Frédérique Magdinier 1 Béatrice Horard 1 Pascal Barbry 2 Rainer Waldmann 2 Eric Gilson 1, 3, 5
1 LBMC - Laboratoire de Biologie Moléculaire de la Cellule
2 IPMC - Institut de pharmacologie moléculaire et cellulaire
3 Laboratory of Biology and Pathology of Genomes
4 Dipartimento di Genetica e Microbiologia Adriano Buzzati-Traverso
5 Department of Medical Genetics
Abstract : The study of the proteins that bind to telomeric DNA in mammals has provided a deep understanding of the mechanisms involved in chromosome-end protection. However, very little is known on the binding of these proteins to nontelomeric DNA sequences. The TTAGGG DNA repeat proteins 1 and 2 (TRF1 and TRF2) bind to mammalian telomeres as part of the shelterin complex and are essential for maintaining chromosome end stability. In this study, we combined chromatin immunoprecipitation with high-throughput sequencing to map at high sensitivity and resolution the human chromosomal sites to which TRF1 and TRF2 bind. While most of the identified sequences correspond to telomeric regions, we showed that these two proteins also bind to extratelomeric sites. The vast majority of these extratelomeric sites contains interstitial telomeric sequences (or ITSs). However, we also identified non-ITS sites, which correspond to centromeric and pericentromeric satellite DNA. Interestingly, the TRF-binding sites are often located in the proximity of genes or within introns. We propose that TRF1 and TRF2 couple the functional state of telomeres to the long-range organization of chromosomes and gene regulation networks by binding to extratelomeric sequences.
keyword : telomere TRF1 TRF2 interstitial telomeric sequence satellite DNA
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Article dans une revue
Cell Research, Nature Publishing Group, 2011, 21, pp.1028-1038. 〈10.1038/cr.2011.40〉
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Contributeur : Agnès Ganivet <>
Soumis le : jeudi 18 avril 2013 - 09:59:20
Dernière modification le : mardi 9 octobre 2018 - 15:40:11

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ENS-LYON | UNICE | UNIV-COTEDAZUR | UNIV-LYON1

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Thomas Simonet, Laure-Emmanuelle Saragosi, Claude Philippe, Kevin Lebrigand, Clémentine Schouteden, et al.. The human TTAGGG repeat factors 1 and 2 bind to a subset of interstitial telomeric sequences and satellite repeats. Cell Research, Nature Publishing Group, 2011, 21, pp.1028-1038. 〈10.1038/cr.2011.40〉. 〈ensl-00815016〉

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