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Journal articles
The human TTAGGG repeat factors 1 and 2 bind to a subset of interstitial telomeric sequences and satellite repeats
Abstract : The study of the proteins that bind to telomeric DNA in mammals has provided a deep understanding of the mechanisms involved in chromosome-end protection. However, very little is known on the binding of these proteins to nontelomeric DNA sequences. The TTAGGG DNA repeat proteins 1 and 2 (TRF1 and TRF2) bind to mammalian telomeres as part of the shelterin complex and are essential for maintaining chromosome end stability. In this study, we combined chromatin immunoprecipitation with high-throughput sequencing to map at high sensitivity and resolution the human chromosomal sites to which TRF1 and TRF2 bind. While most of the identified sequences correspond to telomeric regions, we showed that these two proteins also bind to extratelomeric sites. The vast majority of these extratelomeric sites contains interstitial telomeric sequences (or ITSs). However, we also identified non-ITS sites, which correspond to centromeric and pericentromeric satellite DNA. Interestingly, the TRF-binding sites are often located in the proximity of genes or within introns. We propose that TRF1 and TRF2 couple the functional state of telomeres to the long-range organization of chromosomes and gene regulation networks by binding to extratelomeric sequences.
https://hal-ens-lyon.archives-ouvertes.fr/ensl-00815016
Contributor : Agnès Ganivet Connect in order to contact the contributor
Submitted on : Thursday, April 18, 2013 - 9:59:20 AM
Last modification on : Monday, November 30, 2020 - 6:50:04 PM
Contributor : Agnès Ganivet Connect in order to contact the contributor
Submitted on : Thursday, April 18, 2013 - 9:59:20 AM
Last modification on : Monday, November 30, 2020 - 6:50:04 PM
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