Natural single-nucleosome epi-polymorphisms in yeast.

Abstract : Epigenomes commonly refer to the sequence of presence/absence of specific epigenetic marks along eukaryotic chromatin. Complete histone-borne epigenomes have now been described at single-nucleosome resolution from various organisms, tissues, developmental stages, or diseases, yet their intra-species natural variation has never been investigated. We describe here that the epigenomic sequence of histone H3 acetylation at Lysine 14 (H3K14ac) differs greatly between two unrelated strains of the yeast Saccharomyces cerevisiae. Using single-nucleosome chromatin immunoprecipitation and mapping, we interrogated 58,694 nucleosomes and found that 5,442 of them differed in their level of H3K14 acetylation, at a false discovery rate (FDR) of 0.0001. These Single Nucleosome Epi-Polymorphisms (SNEPs) were enriched at regulatory sites and conserved non-coding DNA sequences. Surprisingly, higher acetylation in one strain did not imply higher expression of the relevant gene. However, SNEPs were enriched in genes of high transcriptional variability and one SNEP was associated with the strength of gene activation upon stimulation. Our observations suggest a high level of inter-individual epigenomic variation in natural populations, with essential questions on the origin of this diversity and its relevance to gene x environment interactions.
Type de document :
Article dans une revue
PLoS Genetics, Public Library of Science, 2010, 6 (4), pp.e1000913. 〈10.1371/journal.pgen.1000913〉
Liste complète des métadonnées

Littérature citée [55 références]  Voir  Masquer  Télécharger
Contributeur : Gaël Yvert <>
Soumis le : mercredi 10 avril 2013 - 14:34:45
Dernière modification le : vendredi 15 février 2019 - 12:20:03
Document(s) archivé(s) le : jeudi 11 juillet 2013 - 04:14:04


Fichiers éditeurs autorisés sur une archive ouverte




Muniyandi Nagarajan, Jean-Baptiste Veyrieras, Maud De Dieuleveult, Hélène Bottin, Steffen Fehrmann, et al.. Natural single-nucleosome epi-polymorphisms in yeast.. PLoS Genetics, Public Library of Science, 2010, 6 (4), pp.e1000913. 〈10.1371/journal.pgen.1000913〉. 〈ensl-00811500〉



Consultations de la notice


Téléchargements de fichiers