CD10 and ICOS expression by multiparametric flow cytometry in angioimmunoblastic T-cell lymphoma

Abstract : Angioimmunoblastic T-cell lymphoma is immunologically defined by the expression of CD10 and the follicular helper T cell (TFH) markers such as CXCL13, programmed death-1 (PD-1) and inducible T-cell costimulator (ICOS). This TFH profile has been mainly reported by immunohistochemistry. Here, using multiparametric flow cytometry, the relevance of ICOS and PD-1 to angioimmunoblastic T-cell lymphoma diagnosis was evaluated in lymph node (n=15) as well as in peripheral blood (n=13) among a series of 28 angioimmunoblastic T-cell lymphoma cases, in addition to the CD10 expression (available in 26 lymph node and 15 peripheral blood specimens). In this series, CD10 expression was present in 23/26 (88%) lymph node and in 12/15 (80%) peripheral blood cases and ICOS in 13/15 (87%) lymph node and in 6/13 (47%) peripheral blood cases, whereas neither significant CD10 nor ICOS T cells were identified in the control group (lymph nodes with reactive hyperplasia=10, peripheral blood of healthy donors=15). PD-1 expression was less informative as observed in both angioimmunoblastic T-cell lymphoma and control cases. The multiparametric approach allowed us to confirm the frequent blood dissemination in angioimmunoblastic T-cell lymphoma and to show that circulating neoplastic T cells correspond more often to a CD10-positive subset than to an ICOS-positive subset. Consequently, if ICOS constitutes an additional feature for the diagnosis of angioimmunoblastic T-cell lymphoma, it appears less sensitive than CD10 expression for the detection of circulating neoplastic T cells.10.1038
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Article dans une revue
Modern Pathology, Nature Publishing Group: Open Access Hybrid Model Option B, 2011, 24, pp.993-1003. 〈10.1038〉
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Contributeur : Agnès Ganivet <>
Soumis le : lundi 8 avril 2013 - 11:08:24
Dernière modification le : jeudi 8 février 2018 - 11:09:30

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  • HAL Id : ensl-00808927, version 1
  • DOI : 10.1038

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Lucile Baseggio, Alexandra Traverse-Glehen, Françoise Berger, Martine Ffrench, Laurent Jallades, et al.. CD10 and ICOS expression by multiparametric flow cytometry in angioimmunoblastic T-cell lymphoma. Modern Pathology, Nature Publishing Group: Open Access Hybrid Model Option B, 2011, 24, pp.993-1003. 〈10.1038〉. 〈ensl-00808927〉

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