CD10 and ICOS expression by multiparametric flow cytometry in angioimmunoblastic T-cell lymphoma

Lucile Baseggio 1, 2 Alexandra Traverse-Glehen 2, 3 Françoise Berger 2, 3 Martine Ffrench 1, 2 Laurent Jallades 1, 2 Dominique Morel 1, 2 Ghislaine Goedert 1, 2 Jean-Pierre Magaud 1, 2 Gilles Salles 2, 4 Pascale Felman 1, 2
1 Laboratoire d'Hématologie Cellulaire
2 LBMC - Laboratoire de Biologie Moléculaire de la Cellule
3 Service d'Anatomie Pathologique
4 Service d'Hématologie
Abstract : Angioimmunoblastic T-cell lymphoma is immunologically defined by the expression of CD10 and the follicular helper T cell (TFH) markers such as CXCL13, programmed death-1 (PD-1) and inducible T-cell costimulator (ICOS). This TFH profile has been mainly reported by immunohistochemistry. Here, using multiparametric flow cytometry, the relevance of ICOS and PD-1 to angioimmunoblastic T-cell lymphoma diagnosis was evaluated in lymph node (n=15) as well as in peripheral blood (n=13) among a series of 28 angioimmunoblastic T-cell lymphoma cases, in addition to the CD10 expression (available in 26 lymph node and 15 peripheral blood specimens). In this series, CD10 expression was present in 23/26 (88%) lymph node and in 12/15 (80%) peripheral blood cases and ICOS in 13/15 (87%) lymph node and in 6/13 (47%) peripheral blood cases, whereas neither significant CD10 nor ICOS T cells were identified in the control group (lymph nodes with reactive hyperplasia=10, peripheral blood of healthy donors=15). PD-1 expression was less informative as observed in both angioimmunoblastic T-cell lymphoma and control cases. The multiparametric approach allowed us to confirm the frequent blood dissemination in angioimmunoblastic T-cell lymphoma and to show that circulating neoplastic T cells correspond more often to a CD10-positive subset than to an ICOS-positive subset. Consequently, if ICOS constitutes an additional feature for the diagnosis of angioimmunoblastic T-cell lymphoma, it appears less sensitive than CD10 expression for the detection of circulating neoplastic T cells.10.1038
Keywords : angioimmunoblastic T-cell lymphoma CD10 circulating neoplastic T cells ICOS multiparametric flow cytometry PD-1
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Journal articles
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Contributor : Agnès Ganivet <>
Submitted on : Monday, April 8, 2013 - 11:08:24 AM
Last modification on : Wednesday, November 20, 2019 - 2:36:49 AM

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  • HAL Id : ensl-00808927, version 1
  • DOI : 10.1038

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Lucile Baseggio, Alexandra Traverse-Glehen, Françoise Berger, Martine Ffrench, Laurent Jallades, et al.. CD10 and ICOS expression by multiparametric flow cytometry in angioimmunoblastic T-cell lymphoma. Modern Pathology, Nature Publishing Group: Open Access Hybrid Model Option B, 2011, 24, pp.993-1003. ⟨10.1038⟩. ⟨ensl-00808927⟩

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