CD10 and ICOS expression by multiparametric flow cytometry in angioimmunoblastic T-cell lymphoma - Archive ouverte HAL Access content directly
Journal Articles Modern Pathology Year : 2011

CD10 and ICOS expression by multiparametric flow cytometry in angioimmunoblastic T-cell lymphoma

(1, 2) , (2, 3) , (2, 3) , (1, 2) , (1, 2) , (1, 2) , (1, 2) , (1, 2) , (2, 4) , (1, 2)
1
2
3
4

Abstract

Angioimmunoblastic T-cell lymphoma is immunologically defined by the expression of CD10 and the follicular helper T cell (TFH) markers such as CXCL13, programmed death-1 (PD-1) and inducible T-cell costimulator (ICOS). This TFH profile has been mainly reported by immunohistochemistry. Here, using multiparametric flow cytometry, the relevance of ICOS and PD-1 to angioimmunoblastic T-cell lymphoma diagnosis was evaluated in lymph node (n=15) as well as in peripheral blood (n=13) among a series of 28 angioimmunoblastic T-cell lymphoma cases, in addition to the CD10 expression (available in 26 lymph node and 15 peripheral blood specimens). In this series, CD10 expression was present in 23/26 (88%) lymph node and in 12/15 (80%) peripheral blood cases and ICOS in 13/15 (87%) lymph node and in 6/13 (47%) peripheral blood cases, whereas neither significant CD10 nor ICOS T cells were identified in the control group (lymph nodes with reactive hyperplasia=10, peripheral blood of healthy donors=15). PD-1 expression was less informative as observed in both angioimmunoblastic T-cell lymphoma and control cases. The multiparametric approach allowed us to confirm the frequent blood dissemination in angioimmunoblastic T-cell lymphoma and to show that circulating neoplastic T cells correspond more often to a CD10-positive subset than to an ICOS-positive subset. Consequently, if ICOS constitutes an additional feature for the diagnosis of angioimmunoblastic T-cell lymphoma, it appears less sensitive than CD10 expression for the detection of circulating neoplastic T cells.10.1038
Not file

Dates and versions

ensl-00808927 , version 1 (08-04-2013)

Identifiers

  • HAL Id : ensl-00808927 , version 1

Cite

Lucile Baseggio, Alexandra Traverse-Glehen, Françoise Berger, Martine Ffrench, Laurent Jallades, et al.. CD10 and ICOS expression by multiparametric flow cytometry in angioimmunoblastic T-cell lymphoma. Modern Pathology, 2011, 24, pp.993-1003. ⟨ensl-00808927⟩
53 View
0 Download

Share

Gmail Facebook Twitter LinkedIn More