Cell cycle regulated expression of NCoR might control cyclic expression of androgen responsive genes in an immortalized prostate cell line

D.M. Altintas 1 V. Vlaeminck 1, 2 Dimitar Angelov 3 Stefan Dimitrov 4 Jacques Samarut 1, 2
1 IGFL - Institut de Génomique Fonctionnelle de Lyon
2 Service d'Urologie
3 LBMC - Laboratoire de Biologie Moléculaire de la Cellule
4 INSERM U823 - Institut d'oncologie/développement Albert Bonniot de Grenoble
Abstract : In this work we have studied the mechanisms of regulation of expression of androgen receptor (AR) target genes. We have used an immortalized non-tumorigenic prostate cell line RWPE-1-ARtag constitutively expressing an exogenous AR as a model. We observed that all studied AR target genes exhibited a specific expression during the G1 phase of the cell cycle despite the constitutive expression of AR. Importantly, we found that the expression of NCoR, an AR co-repressor, was downregulated during the G1 phase and expressed as mRNA and protein specifically during the S phase. The role of NCoR in repressing androgen-induced expression of AR target genes in S phase was further demonstrated by altering expression of NCoR during the cell cycle through knockdown or induced overexpression. Using two alternative techniques we show that AR binds directly to target DNA in the chromatin only during the G1 phase. These data support the hypothesis that NCoR might control a cell cycle dependent regulation of expression AR target genes in prostate cells.
Keywords : Androgen receptor NCoR Chromatin Gene expression Cell cycle
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Journal articles
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https://hal-ens-lyon.archives-ouvertes.fr/ensl-00808297
Contributor : Agnès Ganivet <>
Submitted on : Friday, April 5, 2013 - 11:06:55 AM
Last modification on : Thursday, February 7, 2019 - 5:04:08 PM

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  • HAL Id : ensl-00808297, version 1
  • DOI : 10.1016

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ENS-LYON | U823 | UGA | UNIV-POITIERS | INRA | UNIV-LYON1

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D.M. Altintas, V. Vlaeminck, Dimitar Angelov, Stefan Dimitrov, Jacques Samarut. Cell cycle regulated expression of NCoR might control cyclic expression of androgen responsive genes in an immortalized prostate cell line. Molecular and Cellular Endocrinology, Elsevier, 2011, 332 (1-2), pp.149-162. ⟨10.1016⟩. ⟨ensl-00808297⟩

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