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Novel Roles of Caenorhabditis elegans Heterochromatin Protein HP1 and Linker Histone in the Regulation of Innate Immune Gene Expression

Maja Studencka 1 Anne Konzer 2 Gaël Moneron 1 Dirk Wenzel 3 Lennart Opitz 4 Cécile Bedet 5 Marcus Krüger 2 Stefan W. Hell 1 Jacek R. Wisnieski 6 Henning Schmidt 7 Francesca Palladino 5 Ekkehard Schulze 8 Monika Jedrusik-Bode 1
1 MPI-BPC - Max Planck Institute for Biophysical Chemistry
2 MPI-HLR - Max Planck Institute for Heart and Lung Research
3 Electron Microscopy Group
4 DNA Microarray Facility
5 LBMC - Laboratoire de Biologie Moléculaire de la Cellule
6 Department of Proteomics and Signal Transduction
MPIB - Max Planck Institute of Biochemistry
7 Institute of Genetics
8 Bioinformatics and Molecular Genetics
Abstract : Linker histone (H1) and heterochromatin protein 1 (HP1) are essential components of heterochromatin which contribute to the transcriptional repression of genes. It has been shown that the methylation mark of vertebrate histone H1 is specifically recognized by the chromodomain of HP1. However, the exact biological role of linker histone binding to HP1 has not been determined. Here, we investigate the function of the Caenorhabditis elegans H1 variant HIS-24 and the HP1-like proteins HPL-1 and HPL-2 in the cooperative transcriptional regulation of immune-relevant genes. We provide the first evidence that HPL-1 interacts with HIS-24 monomethylated at lysine 14 (HIS-24K14me1) and associates in vivo with promoters of genes involved in antimicrobial response. We also report an increase in overall cellular levels and alterations in the distribution of HIS-24K14me1 after infection with pathogenic bacteria. HIS-24K14me1 localization changes from being mostly nuclear to both nuclear and cytoplasmic in the intestinal cells of infected animals. Our results highlight an antimicrobial role of HIS-24K14me1 and suggest a functional link between epigenetic regulation by an HP1/H1 complex and the innate immune system in C. elegans.
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Journal articles
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https://hal-ens-lyon.archives-ouvertes.fr/ensl-00807739
Contributor : Agnès Ganivet <>
Submitted on : Thursday, April 4, 2013 - 11:44:02 AM
Last modification on : Thursday, August 20, 2020 - 12:00:17 PM

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  • HAL Id : ensl-00807739, version 1
  • DOI : 10.1128

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ENS-LYON | CNRS | UNIV-LYON1 | UDL

Citation

Maja Studencka, Anne Konzer, Gaël Moneron, Dirk Wenzel, Lennart Opitz, et al.. Novel Roles of Caenorhabditis elegans Heterochromatin Protein HP1 and Linker Histone in the Regulation of Innate Immune Gene Expression. Molecular and Cellular Biology, American Society for Microbiology, 2012, 32 (2), pp.251-265. ⟨10.1128⟩. ⟨ensl-00807739⟩

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