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Journal articles
Novel Roles of Caenorhabditis elegans Heterochromatin Protein HP1 and Linker Histone in the Regulation of Innate Immune Gene Expression
Maja Studencka
1
Anne Konzer
2
Gaël Moneron
1
Dirk Wenzel
3
Lennart Opitz
4
Cécile Bedet
5
Marcus Krüger
2
Stefan W. Hell
1
Jacek R. Wisnieski
6
Henning Schmidt
7
Francesca Palladino
5
Ekkehard Schulze
8
Monika Jedrusik-Bode
1
Lennart Opitz 4
Author
Cécile Bedet 5
Author IdHAL : cbedet
Marcus Krüger 2
Author
Stefan W. Hell 1
Author
Jacek R. Wisnieski 6
Author
Henning Schmidt 7
Author
Francesca Palladino 5
Author
Ekkehard Schulze 8
Author
Monika Jedrusik-Bode 1
Author
1
MPI-BPC - Max Planck Institute for Biophysical Chemistry (Am Faßberg 11, 37077 Göttingen - Germany)
- Max-Planck-Gesellschaft (Jägerstrasse, 10-11, D-10117 Berlin - Germany)
2
MPI-HLR - Max Planck Institute for Heart and Lung Research (Ludwigstr. 43, 61231 Bad Nauheim - Germany)
- Max-Planck-Gesellschaft (Jägerstrasse, 10-11, D-10117 Berlin - Germany)
4
DNA Microarray Facility (Göttingen, Germany - Germany)
- Georg-August-University [Göttingen] (Wilhelmsplatz 1, 37073 Göttingen - Germany)
5
LBMC - Laboratoire de Biologie Moléculaire de la Cellule (46 allée d'Italie 69364 Lyon Cedex 07 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1210 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR5161 (France)
- ENS Lyon - École normale supérieure - Lyon (15 parvis René Descartes - BP 7000 - 69342 Lyon Cedex 07 - France)
- UCBL - Université Claude Bernard Lyon 1 (43, boulevard du 11 novembre 1918, 69622 Villeurbanne cedex - France)
- Université de Lyon (92 rue Pasteur - CS 30122, 69361 Lyon Cedex 07 - France)
6
Department of Proteomics and Signal Transduction (Max Planck Institute of Biochemistry, 82152 Martinsried - Germany)
- MPIB - Max Planck Institute of Biochemistry (Am Klopferspitz 18, 82152 Martinsried, Germany - Germany)
- Max-Planck-Gesellschaft (Jägerstrasse, 10-11, D-10117 Berlin - Germany)
7
Institute of Genetics (University of Braunschweig, Braunschweig, Germany - Germany)
- Technische Universität Braunschweig = Technical University of Braunschweig [Braunschweig] (38092 Braunschweig - Germany)
8
Bioinformatics and Molecular Genetics (Albert-Ludwigs University, Faculty of Biology, Institute for Biology 3, Freiburg, Germany - Germany)
- University of Freiburg [Freiburg] (Friedrichstr. 39 79098 Freiburg - Germany)
Abstract : Linker histone (H1) and heterochromatin protein 1 (HP1) are essential components of heterochromatin which contribute to the transcriptional repression of genes. It has been shown that the methylation mark of vertebrate histone H1 is specifically recognized by the chromodomain of HP1. However, the exact biological role of linker histone binding to HP1 has not been determined. Here, we investigate the function of the Caenorhabditis elegans H1 variant HIS-24 and the HP1-like proteins HPL-1 and HPL-2 in the cooperative transcriptional regulation of immune-relevant genes. We provide the first evidence that HPL-1 interacts with HIS-24 monomethylated at lysine 14 (HIS-24K14me1) and associates in vivo with promoters of genes involved in antimicrobial response. We also report an increase in overall cellular levels and alterations in the distribution of HIS-24K14me1 after infection with pathogenic bacteria. HIS-24K14me1 localization changes from being mostly nuclear to both nuclear and cytoplasmic in the intestinal cells of infected animals. Our results highlight an antimicrobial role of HIS-24K14me1 and suggest a functional link between epigenetic regulation by an HP1/H1 complex and the innate immune system in C. elegans.
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Journal articles
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https://hal-ens-lyon.archives-ouvertes.fr/ensl-00807739
Contributor : Agnès Ganivet Connect in order to contact the contributor
Submitted on : Thursday, April 4, 2013 - 11:44:02 AM
Last modification on : Friday, November 19, 2021 - 12:42:03 PM
Contributor : Agnès Ganivet Connect in order to contact the contributor
Submitted on : Thursday, April 4, 2013 - 11:44:02 AM
Last modification on : Friday, November 19, 2021 - 12:42:03 PM
Identifiers
- HAL Id : ensl-00807739, version 1
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ENS-LYON | CNRS | UNIV-LYON1 | UDL
Citation
Maja Studencka, Anne Konzer, Gaël Moneron, Dirk Wenzel, Lennart Opitz, et al.. Novel Roles of Caenorhabditis elegans Heterochromatin Protein HP1 and Linker Histone in the Regulation of Innate Immune Gene Expression. Molecular and Cellular Biology, American Society for Microbiology, 2012, 32 (2), pp.251-265. ⟨ensl-00807739⟩
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