CKIP-1 regulates mammalian and zebrafish myoblast fusion

Dominique Baas 1 Sabine Caussanel-Boude 1 Alexandre Guiraud 1 Frederico Calhabeu 1 Emilie Delaune 1 Fanny Pilot 1 Emilie Chopin 1 Irma Machuca-Gayet 2 Aurélia Vernay 1 Stéphanie Bertrand 3 Jean-François Rual 4 Pierre Jurdic 2 David E. Hill 4 Marc Vidal 4 Laurent Schaeffer 1 Evelyne Goillot 1
1 LBMC - Laboratoire de Biologie Moléculaire de la Cellule
2 IGFL - Institut de Génomique Fonctionnelle de Lyon
3 OOB - Observatoire océanologique de Banyuls
4 CCSB - Center for Cancer Systems Biology
Abstract : Multinucleated muscle fibres arise by fusion of precursor cells called myoblasts. We previously showed that CKIP-1 ectopic expression in C2C12 myoblasts increased cell fusion. In this work, we report that CKIP-1 depletion drastically impairs C2C12 myoblast fusion in vitro and in vivo during zebrafish muscle development. Within developing fast-twich myotome, Ckip-1 localises at the periphery of fast precursor cells, closed to the plasma membrane. Unlike wild-type myoblasts that form spatially arrayed multinucleated fast myofibres, Ckip-1-deficient myoblasts show a drastic reduction in fusion capacity. A search for CKIP-1 binding partners identified the ARPC1 subunit of Arp2/3 actin nucleation complex essential for myoblast fusion. We demonstrate that CKIP-1, through binding to plasma membrane phosphoinositides via its PH domain, regulates cell morphology and lamellipodia formation by recruiting the Arp2/3 complex at the plasma membrane. These results establish CKIP-1 as a regulator of cortical actin that recruits the Arp2/3 complex at the plasma membrane essential for muscle precursor elongation and fusion.
Keywords : CKIP-1 Arp2/3 Actin cytoskeleton Myoblast fusion Zebrafish muscle development
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Journal articles
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https://hal-ens-lyon.archives-ouvertes.fr/ensl-00807509
Contributor : Agnès Ganivet <>
Submitted on : Wednesday, April 3, 2013 - 4:41:14 PM
Last modification on : Wednesday, November 20, 2019 - 2:54:15 AM

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  • HAL Id : ensl-00807509, version 1
  • DOI : 10.1242

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ENS-LYON | UPMC | CNRS | AGROPOLIS | INRA | UNIV-LYON1 | SORBONNE-UNIVERSITE | SU-SCIENCES | UMS-2348 | UDL

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Dominique Baas, Sabine Caussanel-Boude, Alexandre Guiraud, Frederico Calhabeu, Emilie Delaune, et al.. CKIP-1 regulates mammalian and zebrafish myoblast fusion. Journal of Cell Science, Company of Biologists, 2012, 125, pp.3790-3800. ⟨10.1242⟩. ⟨ensl-00807509⟩

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