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Journal articles
CKIP-1 regulates mammalian and zebrafish myoblast fusion
Dominique Baas
1
Sabine Caussanel-Boude
1
Alexandre Guiraud
1
Frederico Calhabeu
1
Emilie Delaune
1
Fanny Pilot
1
Emilie Chopin
1
Irma Machuca-Gayet
2
Aurélia Vernay
1
Stéphanie Bertrand
3
Jean-François Rual
4
Pierre Jurdic
2
David E. Hill
4
Marc Vidal
4
Laurent Schaeffer
1
Evelyne Goillot
1
Dominique Baas 1
Author
Sabine Caussanel-Boude 1
Author
Alexandre Guiraud 1
Author
Frederico Calhabeu 1
Author
Emilie Delaune 1
Author
Fanny Pilot 1
Author
Emilie Chopin 1
Author
Irma Machuca-Gayet 2
Author IdHAL : irma-machuca-gayet ORCID : https://orcid.org/0000-0002-3010-9774
Aurélia Vernay 1
Author
Stéphanie Bertrand 3
Author IdHAL : stephaniebertrandescriva ORCID : https://orcid.org/0000-0002-0689-0126
Jean-François Rual 4
Author
Pierre Jurdic 2
Author
1
LBMC - Laboratoire de Biologie Moléculaire de la Cellule (46 allée d'Italie 69364 Lyon Cedex 07 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1210 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR5161 (France)
- ENS Lyon - École normale supérieure - Lyon (15 parvis René Descartes - BP 7000 - 69342 Lyon Cedex 07 - France)
- UCBL - Université Claude Bernard Lyon 1 (43, boulevard du 11 novembre 1918, 69622 Villeurbanne cedex - France)
- Université de Lyon (92 rue Pasteur - CS 30122, 69361 Lyon Cedex 07 - France)
2
IGFL - Institut de Génomique Fonctionnelle de Lyon (Ecole Normale Supérieure de Lyon 46 allée d'Italie 69364 Lyon CEDEX07 - France)
- ENS Lyon - École normale supérieure - Lyon (15 parvis René Descartes - BP 7000 - 69342 Lyon Cedex 07 - France)
- INRA - Institut National de la Recherche Agronomique : UA1288 (France)
- CNRS - Centre National de la Recherche Scientifique : UMR5242 (France)
- UCBL - Université Claude Bernard Lyon 1 (43, boulevard du 11 novembre 1918, 69622 Villeurbanne cedex - France)
- Université de Lyon (92 rue Pasteur - CS 30122, 69361 Lyon Cedex 07 - France)
3
OOB - Observatoire océanologique de Banyuls (LABORATOIRE ARAGO BP 44 66651 BANYULS SUR MER CEDEX - France)
- UPMC - Université Pierre et Marie Curie - Paris 6 (4 place Jussieu - 75005 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMS2348 (France)
4
CCSB - Center for Cancer Systems Biology (450 Brookline Ave., Boston, MA 02215 - United States)
- Dana-Farber Cancer Institute [Boston] (450 Brookline Ave. Boston, MA 02215 - United States)
- Department of Cancer Biology (France)
Abstract : Multinucleated muscle fibres arise by fusion of precursor cells called myoblasts. We previously showed that CKIP-1 ectopic expression in C2C12 myoblasts increased cell fusion. In this work, we report that CKIP-1 depletion drastically impairs C2C12 myoblast fusion in vitro and in vivo during zebrafish muscle development. Within developing fast-twich myotome, Ckip-1 localises at the periphery of fast precursor cells, closed to the plasma membrane. Unlike wild-type myoblasts that form spatially arrayed multinucleated fast myofibres, Ckip-1-deficient myoblasts show a drastic reduction in fusion capacity. A search for CKIP-1 binding partners identified the ARPC1 subunit of Arp2/3 actin nucleation complex essential for myoblast fusion. We demonstrate that CKIP-1, through binding to plasma membrane phosphoinositides via its PH domain, regulates cell morphology and lamellipodia formation by recruiting the Arp2/3 complex at the plasma membrane. These results establish CKIP-1 as a regulator of cortical actin that recruits the Arp2/3 complex at the plasma membrane essential for muscle precursor elongation and fusion.
Document type :
Journal articles
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Complete list of metadata
https://hal-ens-lyon.archives-ouvertes.fr/ensl-00807509
Contributor : Agnès Ganivet <>
Submitted on : Wednesday, April 3, 2013 - 4:41:14 PM
Last modification on : Wednesday, November 18, 2020 - 3:08:11 PM
Contributor : Agnès Ganivet <>
Submitted on : Wednesday, April 3, 2013 - 4:41:14 PM
Last modification on : Wednesday, November 18, 2020 - 3:08:11 PM
Identifiers
- HAL Id : ensl-00807509, version 1
Collections
ENS-LYON | UPMC | CNRS | AGROPOLIS | INRA | UNIV-LYON1 | SORBONNE-UNIVERSITE | SU-SCIENCES | UMS-2348 | UDL | INRAE
Citation
Dominique Baas, Sabine Caussanel-Boude, Alexandre Guiraud, Frederico Calhabeu, Emilie Delaune, et al.. CKIP-1 regulates mammalian and zebrafish myoblast fusion. Journal of Cell Science, Company of Biologists, 2012, 125, pp.3790-3800. ⟨ensl-00807509⟩
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