Structural correlations and melting of B-DNA fibres

Abstract : Despite numerous attempts, the understanding of the thermal denaturation of DNA is still a challenge due to the lack of structural data at the transition since standard experimental approaches to DNA melting are made in solution and do not provide spatial information. We report a measurement using neutron scattering from oriented DNA fibres to determine the size of the regions that stay in the double-helix conformation as the melting temperature is approached from below. A Bragg peak from the B-form of DNA has been observed as a function of temperature and its width and integrated intensity have bean measured. These results, complemented by a differential calorimetry study of the melting of B DNA fibres as well as electrophoresis and optical observation data, are analysed in terms of a one-dimensional mesoscopic model of DNA.
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Submitted on : Tuesday, June 14, 2011 - 9:49:10 AM
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Andrew Wildes, Nikos Theodorakopoulos, Jessica Valle Orero, Santiago Cuesta-Lopez, Jean-Luc Garden, et al.. Structural correlations and melting of B-DNA fibres. Physical Review E : Statistical, Nonlinear, and Soft Matter Physics, American Physical Society, 2011, 83, pp.061923. ⟨10.1103/PhysRevE.83.061923⟩. ⟨ensl-00600164⟩

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