Structural correlations and melting of B-DNA fibres

Andrew Wildes; Nikos Theodorakopoulos; Jessica Valle Orero; Santiago Cuesta-Lopez; Jean-Luc Garden; Michel Peyrard

Structural correlations and melting of B-DNA fibres

Andrew Wildes ¹ Nikos Theodorakopoulos ^{2, 3} Jessica Valle Orero ^{1, 4} Santiago Cuesta-Lopez ⁴ Jean-Luc Garden ⁵ Michel Peyrard ⁴

1 ILL - Institut Laue-Langevin

2 Theoretical and Physical Chemistry Institute NHRF

3 Fachbereich Physik [Konstanz]

4 Phys-ENS - Laboratoire de Physique de l'ENS Lyon

5 CapTher - Capteurs thermométriques et calorimétrie

NEEL - Institut Néel

Abstract : Despite numerous attempts, the understanding of the thermal denaturation of DNA is still a challenge due to the lack of structural data at the transition since standard experimental approaches to DNA melting are made in solution and do not provide spatial information. We report a measurement using neutron scattering from oriented DNA fibres to determine the size of the regions that stay in the double-helix conformation as the melting temperature is approached from below. A Bragg peak from the B-form of DNA has been observed as a function of temperature and its width and integrated intensity have bean measured. These results, complemented by a differential calorimetry study of the melting of B DNA fibres as well as electrophoresis and optical observation data, are analysed in terms of a one-dimensional mesoscopic model of DNA.

Keywords : DNA thermal denaturation neutron scattering

Document type :

Journal articles

Domain :

Physics [physics] / Condensed Matter [cond-mat] / Statistical Mechanics [cond-mat.stat-mech]

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https://hal-ens-lyon.archives-ouvertes.fr/ensl-00600164
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Submitted on : Tuesday, June 14, 2011 - 9:49:10 AM
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