Structural correlations and melting of B-DNA fibres

Andrew Wildes 1 Nikos Theodorakopoulos 2, 3 Jessica Valle Orero 1, 4 Santiago Cuesta-Lopez 4 Jean-Luc Garden 5 Michel Peyrard 4
1 ILL - Institut Laue-Langevin
2 Theoretical and Physical Chemistry Institute NHRF
3 Fachbereich Physik [Konstanz]
4 Phys-ENS - Laboratoire de Physique de l'ENS Lyon
5 CapTher - Capteurs thermométriques et calorimétrie
NEEL - Institut Néel
Abstract : Despite numerous attempts, the understanding of the thermal denaturation of DNA is still a challenge due to the lack of structural data at the transition since standard experimental approaches to DNA melting are made in solution and do not provide spatial information. We report a measurement using neutron scattering from oriented DNA fibres to determine the size of the regions that stay in the double-helix conformation as the melting temperature is approached from below. A Bragg peak from the B-form of DNA has been observed as a function of temperature and its width and integrated intensity have bean measured. These results, complemented by a differential calorimetry study of the melting of B DNA fibres as well as electrophoresis and optical observation data, are analysed in terms of a one-dimensional mesoscopic model of DNA.
Keywords : neutron scattering thermal denaturation DNA
Type de document :
Article dans une revue
Physical Review E : Statistical, Nonlinear, and Soft Matter Physics, American Physical Society, 2011, 83, pp.061923
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Soumis le : mardi 14 juin 2011 - 09:49:10
Dernière modification le : mercredi 22 août 2018 - 01:03:33
Document(s) archivé(s) le : dimanche 4 décembre 2016 - 23:28:36

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  • HAL Id : ensl-00600164, version 1
  • ARXIV : 1106.2632

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Andrew Wildes, Nikos Theodorakopoulos, Jessica Valle Orero, Santiago Cuesta-Lopez, Jean-Luc Garden, et al.. Structural correlations and melting of B-DNA fibres. Physical Review E : Statistical, Nonlinear, and Soft Matter Physics, American Physical Society, 2011, 83, pp.061923. 〈ensl-00600164〉

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