Adding a New Dimension to DNA Melting Curves.

Santiago Cuesta-Lopez 1 Dimitar Angelov 2 Michel Peyrard 1
1 Phys-ENS - Laboratoire de Physique de l'ENS Lyon
2 LBMC - Laboratoire de Biologie Moléculaire de la Cellule
Abstract : Standard DNA melting curves record the separation of the two strands versus temperature, but they do not provide any information on the location of the opening. We introduce an experimental method which adds a new dimension to the melting curves of short DNA sequences by allowing us to record the degree of opening in several positions along the molecule all at once. This adds the spatial dimension to the melting curves and allows a precise investigation of the role of the base-pair sequence on the fluctuations and denaturation of the DNA double helix. We illustrate the power of the method by investigating the influence of an AT rich region on the fluctuations of neighboring domains.
Keywords : DNA thermal denaturation statistical physics
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Submitted on : Wednesday, August 19, 2009 - 3:28:33 PM
Last modification on : Wednesday, November 20, 2019 - 2:34:40 AM
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Santiago Cuesta-Lopez, Dimitar Angelov, Michel Peyrard. Adding a New Dimension to DNA Melting Curves.. EPL - Europhysics Letters, European Physical Society/EDP Sciences/Società Italiana di Fisica/IOP Publishing, 2009, 87 (4), pp.48009-1-6. ⟨10.1209/0295-5075/87/48009⟩. ⟨ensl-00410290⟩

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