Efficient use of DNA molecular markers to construct industrial yeast strains.

Abstract : Saccharomyces cerevisiae yeast strains exhibit a huge genotypic and phenotypic diversity. Breeding strategies taking advantage of these characteristics would contribute greatly to improving industrial yeasts. Here we mapped and introgressed chromosomal regions controlling industrial yeast properties, such as hydrogen sulphide production, phenolic off-flavor and a kinetic trait (lag phase duration). Two parent strains derived from industrial isolates used in winemaking and which exhibited significant quantitative differences in these traits were crossed and their progeny (50-170 clones) was analyzed for the segregation of these traits. Forty-eight segregants were genotyped at 2212 marker positions using DNA microarrays and one significant locus was mapped for each trait. To exploit these loci, an introgression approach was supervised by molecular markers monitoring using PCR/RFLP. Five successive backcrosses between an elite strain and appropriate segregants were sufficient to improve three trait values. Microarray-based genotyping confirmed that over 95% of the elite strain genome was recovered by this methodology. Moreover, karyotype patterns, mtDNA and tetrad analysis showed some genomic rearrangements during the introgression procedure.
Type de document :
Article dans une revue
FEMS Yeast Research, Oxford University Press (OUP), 2007, pp.1295-1306. 〈10.1111/j.1567-1364.2007.00281.x〉
Liste complète des métadonnées

Littérature citée [45 références]  Voir  Masquer  Télécharger

https://hal-ens-lyon.archives-ouvertes.fr/ensl-00186849
Contributeur : Gaël Yvert <>
Soumis le : lundi 15 septembre 2008 - 08:42:57
Dernière modification le : mardi 12 juin 2018 - 15:58:29
Document(s) archivé(s) le : lundi 12 avril 2010 - 01:57:45

Fichier

Marullo_b_FEMSYeastRes2007.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Philippe Marullo, Gaël Yvert, Marina Bely, Michel Aigle, Denis Dubourdieu. Efficient use of DNA molecular markers to construct industrial yeast strains.. FEMS Yeast Research, Oxford University Press (OUP), 2007, pp.1295-1306. 〈10.1111/j.1567-1364.2007.00281.x〉. 〈ensl-00186849〉

Partager

Métriques

Consultations de la notice

183

Téléchargements de fichiers

176