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Post synaptic chromatin is under neural control at the neuromuscular junction

Abstract : In adult skeletal muscle, the nicotinic acetylcholine receptor (AChR) specifically accumulates at the neuromuscular junction (NMJ), to allow neurotransmission. This clustering is paralleled by a compartmentalization of AChR genes expression to sub synaptic nuclei, which acquire a unique gene expression program and a specific morphology in response to neural cues. Our results demonstrate that neural agrin-dependent reprogramming of myonuclei involves chromatin remodelling, histone hyper acetylation and histone hyper phosphorylation. Activation of AChR genes in subsynaptic nuclei is mediated by the transcription factor GABP. Here we demonstrate that upon activation, GABP recruits the histone acetyl transferase (HAT) p300 on the AChR Ε subunit promoter, whereas it rather recruits the histone deacetylase HDAC1 when the promoter is not activated. Moreover, the HAT activity of p300 is required in vivo for AChR expression. GABP therefore couples chromatin hyperacetylation and AChR activation by neural factors in subsynaptic nuclei.
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Contributor : Laurent Schaeffer Connect in order to contact the contributor
Submitted on : Thursday, October 25, 2007 - 9:39:29 AM
Last modification on : Thursday, January 13, 2022 - 2:20:44 PM


  • HAL Id : ensl-00182189, version 1
  • PRODINRA : 251167



Aymeric Ravel Chapuis, Marie Vandromme, Jean Luc Thomas, Laurent Schaeffer. Post synaptic chromatin is under neural control at the neuromuscular junction. EMBO Journal, EMBO Press, 2007, 26 (4), pp.1117-1128. ⟨ensl-00182189⟩



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