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AFM imaging of SWI/SNF action : mapping the nucleosome remodeling and sliding

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Abstract

We propose a combined experimental (Atomic Force Microscopy) and theoretical study of the structural and dynamical properties of nucleosomes. In contrast to biochemical approaches, this method allows to determine simultaneously the DNA complexed length distribution and nucleosome position in various contexts. First, we show that differences in the nucleo-proteic structure observed between conventional H2A and H2A.Bbd variant nucleosomes induce quantitative changes in the in the length distribution of DNA complexed with histones. Then, the sliding action of remodeling complex SWI/SNF is characterized through the evolution of the nucleosome position and wrapped DNA length mapping. Using a linear energetic model for the distribution of DNA complexed length, we extract the net wrapping energy of DNA onto the histone octamer, and compare it to previous studies.
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Dates and versions

ensl-00141282 , version 1 (12-04-2007)

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  • HAL Id : ensl-00141282 , version 1

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Fabien Montel, Emeline Fontaine, Philippe St-Jean, Martin Castelnovo, Cendrine Faivre-Moskalenko. AFM imaging of SWI/SNF action : mapping the nucleosome remodeling and sliding. 2007. ⟨ensl-00141282⟩
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