The C. elegans HP1 homologue HPL-2 and the LIN-13 zinc finger protein form a complex implicated in vulval development.

Abstract : HP1 proteins are essential components of heterochromatin and contribute to the transcriptional repression of euchromatic genes via the recruitment to specific promoters by corepressor proteins including TIF1 and Rb. The Caenorhabditis elegans HP1 homologue HPL-2 acts in the "synMuv" (synthetic multivulval) pathway, which defines redundant negative regulators of a Ras signaling cascade required for vulval induction. Several synMuv genes encode for chromatin-associated proteins involved in transcriptional regulation, including Rb and components of the Mi-2/NuRD and TIP60/NuA4 chromatin remodeling complexes. Here, we show that HPL-2 physically interacts in vitro and in vivo with the multiple zinc finger protein LIN-13, another member of the synMuv pathway. A variant of the conserved PXVXL motif found in many HP1-interacting proteins mediates LIN-13 binding to the CSD of HPL-2. We further show by in vivo localization studies that LIN-13 is required for HPL-2 recruitment in nuclear foci. Our data suggest that the LIN-13/HPL-2 complex may physically link a subset of the Rb related synMuv proteins to chromatin.
Type de document :
Article dans une revue
Developmental Biology, Elsevier, 2006, 297 (2), pp.308-322. 〈10.1016/j.ydbio.2006.04.474〉
Liste complète des métadonnées

Littérature citée [28 références]  Voir  Masquer  Télécharger

https://hal-ens-lyon.archives-ouvertes.fr/ensl-00132174
Contributeur : Karine Monier <>
Soumis le : jeudi 14 juin 2007 - 10:01:29
Dernière modification le : jeudi 11 janvier 2018 - 06:15:44
Document(s) archivé(s) le : vendredi 21 septembre 2012 - 11:35:22

Fichier

Palladino.pdf
Fichiers produits par l'(les) auteur(s)

Identifiants

Collections

Citation

Vincent Coustham, Cécile Bedet, Karine Monier, Sonia Schott, Marianthi Karali, et al.. The C. elegans HP1 homologue HPL-2 and the LIN-13 zinc finger protein form a complex implicated in vulval development.. Developmental Biology, Elsevier, 2006, 297 (2), pp.308-322. 〈10.1016/j.ydbio.2006.04.474〉. 〈ensl-00132174〉

Partager

Métriques

Consultations de la notice

112

Téléchargements de fichiers

99