DNA methylation promotes Aurora-B-driven phosphorylation of histone H3 in chromosomal subdomains

Abstract : Confinement of enzymatic reactions to nuclear and chromosomal subdomains regulates functional organization of the nucleus. Aurora-B kinase regulates cell cycle dependent phosphorylation of chromosomal substrates through sequential localization to a series of sites on chromosomes and the mitotic spindle. In G2 nuclei, Aurora-B recruitment to heterochromatin restricts histone H3S10 phosphorylation to a domain around centromeres (pericentromeres). However, no intrinsic chromosomal determinants have been implicated in Aurora-B recruitment to interphase pericentromeres. Using cyclin B1 as a cell cycle marker, we found that the great majority of nuclei exhibiting H3S10 phosphorylated foci were positive for cyclin B1, thus revealing that H3S10 phosphorylation arises at pericentromeres during late S phase and persists in G2. By immuno-fluorescent in situ hybridization, Aurora-B and H3S10 phosphorylated foci were found more frequently at larger pericentromeres than at smaller ones, revealing a preferential phosphorylation of pericentromeres, exhibiting a high density of methyl cytosines. Disruption of DNA methylation inhibited pericentromeric Aurora-B targeting and H3S10 phosphorylation in G2 nuclei, thus demonstrating the role of DNA methylation in Aurora-B targeting to pericentromeres. These results favour the idea that DNA methylation maintains a local environment essential for regulating the functional properties of sub-chromosomal domains during S-G2 progression.
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Journal of Cell Science, Company of Biologists, 2007, 120 (1), pp.101-114. 〈10.1242/jcs.03326〉
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Contributeur : Karine Monier <>
Soumis le : mardi 20 février 2007 - 14:49:02
Dernière modification le : samedi 5 mai 2018 - 01:23:31
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Karine Monier, Sandrine Mouradian, Kevin Sullivan. DNA methylation promotes Aurora-B-driven phosphorylation of histone H3 in chromosomal subdomains. Journal of Cell Science, Company of Biologists, 2007, 120 (1), pp.101-114. 〈10.1242/jcs.03326〉. 〈ensl-00132163〉

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