HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

DNA methylation promotes Aurora-B-driven phosphorylation of histone H3 in chromosomal subdomains

Abstract : Confinement of enzymatic reactions to nuclear and chromosomal subdomains regulates functional organization of the nucleus. Aurora-B kinase regulates cell cycle dependent phosphorylation of chromosomal substrates through sequential localization to a series of sites on chromosomes and the mitotic spindle. In G2 nuclei, Aurora-B recruitment to heterochromatin restricts histone H3S10 phosphorylation to a domain around centromeres (pericentromeres). However, no intrinsic chromosomal determinants have been implicated in Aurora-B recruitment to interphase pericentromeres. Using cyclin B1 as a cell cycle marker, we found that the great majority of nuclei exhibiting H3S10 phosphorylated foci were positive for cyclin B1, thus revealing that H3S10 phosphorylation arises at pericentromeres during late S phase and persists in G2. By immuno-fluorescent in situ hybridization, Aurora-B and H3S10 phosphorylated foci were found more frequently at larger pericentromeres than at smaller ones, revealing a preferential phosphorylation of pericentromeres, exhibiting a high density of methyl cytosines. Disruption of DNA methylation inhibited pericentromeric Aurora-B targeting and H3S10 phosphorylation in G2 nuclei, thus demonstrating the role of DNA methylation in Aurora-B targeting to pericentromeres. These results favour the idea that DNA methylation maintains a local environment essential for regulating the functional properties of sub-chromosomal domains during S-G2 progression.
Document type :
Journal articles
Complete list of metadata

Contributor : Karine Monier Connect in order to contact the contributor
Submitted on : Tuesday, February 20, 2007 - 2:49:02 PM
Last modification on : Friday, March 12, 2021 - 4:48:02 PM
Long-term archiving on: : Wednesday, April 7, 2010 - 12:20:15 AM




Karine Monier, Sandrine Mouradian, Kevin Sullivan. DNA methylation promotes Aurora-B-driven phosphorylation of histone H3 in chromosomal subdomains. Journal of Cell Science, Company of Biologists, 2007, 120 (1), pp.101-114. ⟨10.1242/jcs.03326⟩. ⟨ensl-00132163⟩



Record views


Files downloads