Single-cell phenomics reveals intra-species variation of phenotypic noise in yeast. - ENS de Lyon - École normale supérieure de Lyon Accéder directement au contenu
Article Dans Une Revue BMC Systems Biology Année : 2013

Single-cell phenomics reveals intra-species variation of phenotypic noise in yeast.

Résumé

BACKGROUND: Most quantitative measures of phenotypic traits represent macroscopic contributions of large numbers of cells. Yet, cells of a tissue do not behave similarly, and molecular studies on several organisms have shown that regulations can be highly stochastic, sometimes generating diversified cellular phenotypes within tissues. Phenotypic noise, defined here as trait variability among isogenic cells of the same type and sharing a common environment, has therefore received a lot of attention. Given the potential fitness advantage provided by phenotypic noise in fluctuating environments, the possibility that it is directly subjected to evolutionary selection is being considered. For selection to act, phenotypic noise must differ between contemporary genotypes. Whether this is the case or not remains, however, unclear because phenotypic noise has very rarely been quantified in natural populations. RESULTS: Using automated image analysis, we describe here the phenotypic diversity of S. cerevisiae morphology at single-cell resolution. We profiled hundreds of quantitative traits in more than 1,000 cells of 37 natural strains, which represent various geographical and ecological origins of the species. We observed abundant trait variation between strains, with no correlation with their ecological origin or population history. Phenotypic noise strongly depended on the strain background. Noise variation was largely trait-specific (specific strains showing elevated noise for subset of traits) but also global (a few strains displaying elevated noise for many unrelated traits). CONCLUSIONS: Our results demonstrate that phenotypic noise does differ quantitatively between natural populations. This supports the possibility that, if noise is adaptive, microevolution may tune it in the wild. This tuning may happen on specific traits or by varying the degree of global phenotypic buffering.

Domaines

Génétique
Fichier principal
Vignette du fichier
1752-0509-7-54.pdf (688.01 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

ensl-00845319 , version 1 (16-07-2013)

Identifiants

Citer

Gaël Yvert, Shinsuke Ohnuki, Satoru Nogami, Yasutaka Imanaga, Steffen Fehrmann, et al.. Single-cell phenomics reveals intra-species variation of phenotypic noise in yeast.. BMC Systems Biology, 2013, 7 (1), pp.54. ⟨10.1186/1752-0509-7-54⟩. ⟨ensl-00845319⟩
138 Consultations
152 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More